Isolation of Mitochondria

In this lab, we used differential centrifugation to isolate mitochondria and nuclei from cauliflower. We also bill the rate at which a certain molecule is born-again to another molecule and how much a given enzyme piece of ass convert at a given time. Then we go forth get three fractions and find out which fraction has the close mitochondria and which fraction has the purest mitochondria. Nuclei and mitochondria are both organelles that are found at bottom most eukaryotic cells. The nucleus is enclosed by a double membrane nuclear envelope. Inside the nucleus deoxyribonucleic acid is organized into material called chromatin. Nuclei are the central part of the cell, the densest organelle, and considered to be the brain of the cell. Mitochondria are membrane-bound organelles, and like the nucleus have a double membrane. The outer membrane is fairly smooth. But the midland membrane is highly intricate, forming folds called cristae. The cristae greatly increase the inner membranes near area. Mitochondria are cellular power plants because they generate most of the cells sum of adenosine triphosphate (ATP), used as a source of chemical energy. During the lab, we exit not be able to see the mitochondria, so we finished an enzyme assay for succinate dehydrogenase to report the mitochondria.




In preparing three cell fractions, we mandatory to weigh out 30 g. of chilled cauliflower and cut it into atomic pieces. Then grind it up in a chilled howitzer with 30 ml of cold grinding solution. While grinding the cauliflower, we sprinkle some cold sand into the mortar hence poured the solution through 4 layers cheese cloth into a clean cold beaker. Then pour the filtrate into a chilled centrifuge and centrifuge at 1000 x g for 10 minutes at 4°C. During the centrifuge, we prepared 7 cuvettes for enzyme assay. Each cuvette has 0.5 ml of 0.3 M phosphate buffer, 0.5 ml of 0.05 M MgSO4, 0.5 ml of 0.06 M HCl and 4 drops of 0.3 M sodium azide. by and by centrifuge is done, we draw a line on the slideway of the centrifuge tube...If you want to get a full essay, erect it on our website: Ordercustompaper.com



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